ELECTROLYTE

ELECTROLYTE

Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion also More »

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

LIST OF TEST UNDER E/U/CREATININE Sodium (Na) Potassium (K) Chloride (Cl) Bicarbonate (HCO3) Urea (U) Creatinine (Cr) Glucose (Gluc)   ESTIMATION OF SODIUM & POTASSIUM There are three method used for the More »

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASIUM ESTIMATION REAGENT   A STOCK SODIUM CHLORIDE STANDARD (1.0M) COMPOSITION Sodium chloride 45g Distilled water 1litre PREPARATION Dissolved 58.45g of sodium chloride in 20ml of distilled water, make up the solution to More »

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY REAGENT DILUTING FLUID COMPOSITION Glacial acetic acid 100ml Concentrated nitric acid 6.4ml Deionized water 1litre PREPARATION Mixed 100ml of glacial acetic acid with 200ml of deionized More »

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

ESTIMATION OF PLASMA/SERUM BIOCARBONATE REAGENT NORMAL SALINE COMPOSITION Saline                                           1g Distilled water                            100ml PREPARATION Dissolved 1g of Sodium chloride in 100ml of 100ml water 01N (HCL) HYDROCHLORIC ACID (NaOH) SODIUM HYDROXIDE 01N More »

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA Almost half of non proteinous nitrogen substance found in the blood is the constituent of urea. Urea is a product of amino acid that is produce when amino More »

container to be used collect dample for blood urea Nitrogen Test

container to be used collect dample for blood urea Nitrogen Test

SAPMLE USE TO CARRY OUT THE TEST SPECIMEN: whole blood can be used to perform blood urea nitrogen. Since whole blood can be used, then EDTA container can be use to collect More »

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD REAGENT BUFFERED UREASE REAGENT COMPOSITION EDTA (Ethylene diaminetetracetic acid)                     1.0g Urease                                                                           150mg Distilled water                                                              100ml PREPARATION Dissolved 1.0g of EDTA (Ethylene diaminetetracetic acid), and 150mg More »

Laboratory Examination Of Creatinine

Laboratory Examination Of Creatinine

ESTIMATION OF CREATININE Creatinine is a nitrogenous product that is produced when metabolism of creatine in the skeletal and muscle. They are filter by the kidney and excreted in the urine. REAGENT More »

GLOCOMETER AND IT USES

GLOCOMETER AND IT USES

Glucometer can be define as instrument use for measuring the blood sugar level of a patient. The advent of glucometer come as result of scientist try to finding quick way to estimate More »

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

ELECTROLYTE Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion More »

cause of male infertility and laboratory investigation

cause of male infertility and laboratory investigation

seminal fluid analysis Seminal fluid analysis is one of the important investigation of male in fertility in medical laboratory. The often requested for men that have issue of getting a woman pregnant. More »

Effect of alcohol on the body system

Effect of alcohol on the body system

Effect of alcohol on the body system Digestive and endocrine glands: Drinking too much alcohol can cause abnormal activation of digestive enzymes produced by the pancreas. Buildup of these enzymes can lead More »

SEMINAL FLUID ANALYSIS

SEMINAL FLUID ANALYSIS

Practical step of seminal fluid analysis EXAMINATION PARAMETER MACROSCOPIC EXAMINATION Time of arrival Appearances of the specimen Viscosity/liquefaction Volume of the specimen MICROSCOPIC EXAMINATION MOTILITY Quantitative motility Qualitative motility Sperm count HOW More »

 

Category Archives: Biochemistry

Mutarotation of D-glucose

MUTAROTATION When aldehyde is first dissolved in water and the solution is put in optical part so that the surface polarized light passed. The first optical rotation show by sugar slowly change it way until it form a fixed constant

The word ‘Carbohydrates’ define as a group of organic compounds, starting from Simple sugars monosaccharide to complex sugars polysacchacharides,

INTRODUCTION The word ‘Carbohydrates’ define as a group of organic compounds, starting from Simple sugars monosaccharide to complex sugars polysacchacharides, that make up some of the essential structures in the biosphere. Carbohydrates is one of the most available biomolecules on

Property of protein

Properties of Proteins   Proteins are known to have the following major properties:   1.  Proteins have varying molecular weights. The molecular weights of insulin,  haemoglobin and immunoglobulins are 5700, 68000 and 150000 respectively.  2.  Absorption of ultraviolet light. They strongly

STEREOISOMERISM

This is the present of asymmetric carbon atom in any compound. The present of asymmetric carbon atom in a compound give rise to formation of isomer of that compound, such will be identical in composition but differ in partial configuration;

GENERAL PROPERTIES OF GLUCOSE

Glucose is one of the important carbohydrates in the field of bimolecular chemistry. Glucose has a general formula which is; (C6H12O6), with this formula, it renders glucose to be called hexose sugar. In a general properties reference to glucose it

CHIRAL CARBON

Chiral carbon is a carbon in a carbohydrate chain that does not have a repetitive of 2 or more of same atom bind to it chain In the above diagram it will be observed that there is no repetition of any atom attached

CHEMISTRY OF CARBOHYDRATES

Carbohydrate is biochemical substance in living organism. Carbohydrate is found in large number of food, such as sugar, milk, jams potatoes, cereal, bread, etc. Many more of carbohydrate found in the universe is known as biomolecule. All sugar is carbohydrate,

CARBON AND IT PROPERTIES

Carbon is atom is found group 14, in a complete form of periodic table but basically is found in group four when periodic are arranged base on it first twenty  element. carbon exist in two allotrope form which are diamond