Category Archives: Analytical Chemistry

Spectroscopic analysis

Spectroscopic procedure of analysis is by estimating the intensity and wavelength of radiation that is transmitted or absorbed in the spectrophotometry. This step give foundation for sensitivity procedure for determination and quantization. On the basis of determination and quantization of


in our previous topic we discussed on the following; What did you understand by the following terms; (a) Auxochrome (b) bathochromic shift (c) hyperchromic shift? On that topic definition of the words where given so you can click on the

Principle of UV Visible Spectroscopy

Ultraviolet’s spectroscopy The electromagnetic radiation covers a long range of radiations which are broken down into specific areas in accordance to wavelength. The Ultraviolet (UV) vicinity extends from approximately 10 to 380 nm, but the maximum analytically beneficial area is


The Ultraviolet (uv) region extends from about 10 to 380 nm, but the most analytical useful region is from 200 – 380nm, called the near ultraviolet region. Below 200 nm, the air absorbs appreciably and so the instruments are operated

Treatment or Preparation of Sample

Samples brought to the laboratory require further treatment before analysis commences. This is due to the need to convert the sample from the nature in which it exist at the site of sampling to the form in which it can

Gross and Laboratory size Samples

Irrespective of the state of materials to be sampled, the level of heterogeneity, the size, nature and volume of the sample and configuration of the site , the interest of the analyst is to generate gross sample from which laboratory

Types of Analytical Samples

Types of Samples The following are various types of sample that can be collected. (i) Grab Samples: These are single samples collected at specific spot at a siteover a short period of time. These types samples represent a “snap shot”

Sampling techniques

Basically there are two main sampling techniques. (i) Random Sampling: This is a method of sampling in which each item of the population has equal chance of being included in the samples. Random sampling can be from either finite samples

Theory of Sampling

There are certain important steps that naturally precede chemical analysis if the result or data generated is to have any significance. These steps include: (i) Sampling (ii) Production of a homogenous mixture for analysis and (iii) Drying the collected sample.

Gross and Laboratory size Samples

Irespective of the state of materials to be sampled, the level of heterogeneity, the size , nature and volume of the sample and configuration of the site , the interest of the analyst is to develop a gross sample of

Laboratory Sampling Techniques

Sampling techniques On this post we are going to discuss the basic principle of collecting sample for investigation. Sample is the small aliquot taken from a whole sample to represent the entire population. Before sample can be said to represent

Basic principle of sampling

The basic purpose of this post is to introduced student to basic principle of sampling. Sampling can be described as the operations involved in procuring a laboratory size that is a true representative of “a whole lot for a particular

Type of Laboratory sample

There verious type of sample required for laboratory investigation. On this post we going to discus them one after the other. The following are various types of sample that can be collected. Grab Samples: These are single samples collected at


CHROMATOGRAPHY AND IT TYPES Chromatography is an analytical method widely employed in the field of chemistry and biology for separation.  The method is used mostly with a natural occurring substance, because most of this naturally occurring substance are mixture of

Statistical correlation

        Correlation Correlation is statistical tools that are used to study the relationship between the two variable. This studies assist in revealing the magnitude of association between two set of variable. With knowledge of correlation we can

Statistical Student t-Test

Student’s t Test This is the most frequently used tools in statistical analysis for comparing the experimental method with means. This tools assist in analyzing confidence interval that is the range in which the true value might fall within a

Coefficient of Variation

Coefficient of Variation before proceding, if you have not read  Way of expressing precision  i sugest you read it it will give you more understanding of what we duscuss hear. Standard deviation is the correct method of measuring dispersion. The

Way of expressing precision

Way of expressing precision measurement With application of statistics to scientific problem, it has permitted scientist to reject or accept conclusions on the outcome of analysis figures depending on the degree of precision or attached numerical report. Precision can be

Various statistical term definitions

Various statistical term definitions Significant figures: may be define a maximum digit number required to analyze a given value scientifically with a precision measurement. This way is very significant in messaging the exact meaning and status of each digit. The

Prediction and correction of systematic error

There are many way to determine and correct systematic error which are; Composition sample analysis: on this way, the method used should provide a known answers, if the method used is not able to provide that, then the error could

Error in in measuring Analytical chemistry

Introduction to Analytical chemistry As the name implied, analytical chemistry is that field of chemistry that specialized in both qualitative analysis, investigating what constituent of a given sample, and quantitative analysis, investigating what amount of quantities present in a given