Nature of Sample to be collected for chemical test and volume required

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SPECIMENQOUANTITYINVESTIGATIONREMARKS
Blood, Clotted3-5mlAcid phosphate
Blood, Clotted3-5mlAlanine aminotransferase

(SGPT)

Blood, Clotted2-3mlAlbumin
Blood, Clotted3-5mlAlkaline phosphate
Blood, Clotted3-5mlAmylase
Blood, Clotted3-5mlAspartate aminotransferase

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(SGOT)

Blood, Clotted3-5mlTotal bilirubin1-2ml anticoagulated blood for infants. Free of haemolysis
Blood, Clotted1-2mlCalciumFasting specimen. Chemically
Blood, Clotted3-5mlCholesterolPreferably fasting specimen
Blood, Clotted2-3mlCreatinineFree of haemolysis
Blood, Clotted5-10mlElectrolyteFree of haemolysis Anticoagulated (heparinised) blood can be used
Blood, Clotted3-5mlLactate dehydrogenase (LDH)Free of haemolysis
Blood, Clotted3-5mlPhosphorus (inorganic)
Blood, Clotted3-5mlTotal Proteins
Blood, Clotted4-5mlThyroxine
Blood, Clotted3-5mlTriglyceridesFasting specimen. Anticoagulated (EDTA) blood can be used

Blood, Clotted4-5mlUric acidKeep in dark
Blood anticoagulated (heparin)

Blood anticoagulated (fluoride oxalate)

4-5ml

1-2ml

Blood gass

Glucose

No air bubble in the specimen analyse promptly

Fasting, post-prandial or random specimen

Blood Anticoagulated (EDTA) or

Blood anticoagulated (fluoride oxalate)

1-2mlUrea
Urine10-20mlRoutine AnalysisFor specific gravity, collect 50-60ml of urine
Urine24 hour specimen in a bottle containing 20ml conc. HCLCalccium
Urine24 hour specimenCreatinineFree of Preservative
Urine24 hour specimenProtein quantitative
FaecesSmall portion each day for 3 days

Consecutively

Occult blood
Cerebrospinal fluid1-2mlBiochemical analysis

(protein, glucose)

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