This term simply mean killing of all form of lives that is present in an equipment. In general term sterilization simply means killing entire form of lives which is found in a sample, equipment or in an environment. But in the study of bacteria, these words, is used as a guideline in achieving aseptic environment i.e. killing a bacteria in a contaminated sample and a technique applied for achieving the exclusion of unwanted microorganism in a culture media, these are usually achieved through filtration technique.
The techniques used to achieve sterilization in the laboratory are group into the following;