LOEFFER ALKALINE METHYLINE BLUE
This is a basic dyes used for examination of microorganism structure in the microbiology laboratory, these method is usually appear to smear from cultures. The solution is slower than that of methylene blue in such way lead to slow performance like that of polychromatic staining.
Methylene blue in 1.5% in 95% alcohol 300ml
Potassium hydroxide 10ml
Distilled water 990ml
Measure potassium hydroxide solution into distilled water and mix with methylene blue solution thoroughly filter date and record.
Apply the solution for 30 second, wash with water blot carefully and dry with gentle heat.
Examine with microscope.