preparation Liquid and solid cultural sample for microscopic Examination

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LIQUID MEDIA SPECIMEN PREPARATION STEP

  1. Sterilized wire loop by passing through flame, allowed it to red hot bring it out and allowed it to cool before picking a colony
  2. Take a loop full of culture; transfer to a clean grease free glass slide spread the sample with the loop to form a thick film of a fluid.
  3. sterilized the wire loop
  4. Leave the film to air dry without heating and pass through the flame 3 time to kill the bacteria and fixed it to a slide.
  5. the slide should be allowed to cool
  6. stain with the right stain
  7. examine with microscopy, by microscopic procedure.

SOLID MEDIA SPECIMEN PREPARATION STEP

  1. sterilized the wire loop
  2. place a drop of distilled water on a grease free clean slide
  3. sterilized the loop
  4. with wire loop transfer a loopful colony to be examine on a slide
  5. mix it with the water to give you a uniformed films, sterilized the wire loop
  6. Allowed the film to air dry and fix with the appropriate stain and take it to microscope for examination via microscopic procedure.
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