Bacteriophage

Bacteriophage

Bacteriophage is the term referred to a virus that used bacteria as their host. Bacteria creates two types of toxin which are: Exotoxin Edotoxin Exotoxin A component of toxin obtained from gram More »

VARIOUS WATER USED FOR MEDIA PREPATION

VARIOUS WATER USED FOR MEDIA PREPATION

PEPTONE WATER This is water that is use for examination of water sugar called peptone water sugar. The water is for preparation of sugar media and also used for cultivation of vibro More »

TYPE OF MEDIA AND IT COMPOSITION

TYPE OF MEDIA AND IT COMPOSITION

Nutrient Broth Nutrient Broth: these media is used for growing fastidious bacteria that is an organism that is not too difficult to cultivate. These media are basal media, in such case use More »

Culture production evolution

Culture production evolution

On the origin of bacteriology, the culture media used by then are ox Heart or Calf brain. But with the advancement of knowledge of bacteriology, this type of media is drastically disappearing More »

FAULT IN CULTURE MEDIA

FAULT IN CULTURE MEDIA

There are varieties of fault found in culture media mostly when preparation of dehydrated media is in progress such as; Inappropriate weigh, inaccurate measure of water: this problem take place as a More »

Culture production evolution

Culture production evolution

On the origin of bacteriology, the culture media used by then are ox Heart or Calf brain. But with the advancement of knowledge of bacteriology, this type of media is drastically disappearing More »

HOW TO ISOLATE A SINGLE COLONY

HOW TO ISOLATE A SINGLE COLONY

For isolation of a single colony, there are step required to inoculate the petri dish before the mission can be accomplished. The steps are; Sterile wire loop, take a loopful specimen and More »

Microbiology cultural techniques

Microbiology cultural techniques

TUBE CULTURE TECHNIQUE This culture technique involved the following; stop culture, deep culture and roll culture. Slope Culture: several test technique is used to classified micro organism preferably on solid medium, slope More »

Use of petri dish for culturering

Use of petri dish for culturering

In other to study the features and the characteristics of micro organism colonies, petri dish is the apex apparatus used for the task. Petri is shallow, flat and have a large surface More »

HOW TO STORE PREPEARD MEDIA

HOW TO STORE PREPEARD MEDIA

required environment to store prepared media Just like order substance in the laboratory like the way we store chemicals, which chemical have there own environment which they can be stored, so do More »

TYPE OF MICROBIOLOGY CULTURE MEDIA

TYPE OF MICROBIOLOGY CULTURE MEDIA

Media is group in to two which are; Liquid media, and solid media. Liquid media: as the name implied, these types of media are liquid in nature, they are used to culture More »

HYDROGEN ION CONCENTRATION CULTURE MEDIA

HYDROGEN ION CONCENTRATION CULTURE MEDIA

HYDROGEN ION CONCENTRATION This is the acidic or basic content required by the micro organism to growth. The PH of bacteria varies, some required acidic environment before they can growth, some other More »

CRETERIA OF CULTURE MEDIA

CRETERIA OF CULTURE MEDIA

These mean the status which the media must ascertain before they can support the growth of bacteria, moisture, carbon, and nitrogen substance are very essential for artificial media to meet there requirement More »

USE OF CULTURE MEDIA

USE OF CULTURE MEDIA

Culture media Cultue media: is a simple and artificial food which micro organism required for it growth. Before organism can be study, it must first of all be culture and isolated, this More »

CONTROL OF FILTRATION PROCESS

CONTROL OF FILTRATION PROCESS

CONTROL OF FILTRATION PROCESS This simply means the process where filtration is directed to achieve a satisfactory result of filtration. In filtration there are some challenge face, this can be overcome by More »

STERILIZATION BY FILTRATION

STERILIZATION BY FILTRATION

STERILIZATION BY FILTRATION The first approach on how to purify water where carried out by allowing water to pass through a bed of sand gavel and cinders. But as the knowledge of More »

GASEOUS STERILIZATION

GASEOUS STERILIZATION

GASEOUS STERILIZATION There are some chemical substances which are gaseous in nature that are used in the laboratory for sterilization i.e formaldehyde and ethylene oxide. This substance is used as an alternative More »

CHEMICAL AGENT FOR STERILIZATION

CHEMICAL AGENT FOR STERILIZATION

CHEMICAL AGENT There are several chemical agent is used for disinfection. Disinfectant is a chemical substance that destroyed microorganism in an inanimate object. The chemical agent that kill germs or inhibit it More »

LOW TEMPERATURE STERILIZATION

LOW TEMPERATURE STERILIZATION

LOW TEMPERATURE STERILIZATION Biological fluid also sterilized by heating it with water bath at a temperature of 56ºC for period of one hour. This process required continuous sterilization in a daily interval More »

CHEMICAL STERILIZATION

CHEMICAL STERILIZATION

Chemical sterilization is one of the frequently used methods of sterilization in the laboratory and the entire hospital. The ways which chemical are used for sterilization depend on the strength and effect More »

 

Bacteria Metabolism

 METABOLSM

Life is the vital source of all living things, either plant or animals, big or small. Whatever their size may be, life is essentially important to all living things.

If any organism must survived, it must be able to respond to change in it environment, must be able to feed, able to exchange gas with it environment, and reproduction, these are all essential part of life.

So also the bacteria possess all these characteristic of life, in other to obtain a normal growth of bacteria in the laboratory, nutrient media is necessary. The knowledge of the metabolism is important. Metabolism in bacteria is as a result of chemical interaction that take place spontaneously in the bacteria, but the reaction is speed up to completion in short time by protein substance called enzyme. Reactions that take place do to metabolic reaction are in two processes which are;

READ ALSO  Gram Positive and Negative of Bacteria

Catabolic Reaction:

These reactions occur as a result of broken down of complex substance to simpler one which is used by the bacteria.

Anabolic Reaction:

this reaction result in the formation of complex substance from the simple one in the living organism when the need arise.

In catabolism, energy is needed to bring the reaction to completion. This energy is obtained from broken down of molecular substance. The energy requires may be 1000nm in singles cells for it transfer. For the identification of bacteria, is due to action of enzyme on their specific substrate. As it was stated previously, oxygen is require for the growth of some bacteria, but it is obvious that the effect of oxygen in the growth of bacteria cell depend either the oxygen is used for final hydrogen acceptor in the respiratory processes, which is confer to aerobic respiration. It seem to be that all bacteria require some carbon dioxide (CO2) from the atmosphere and most of the bacteria growth rapidly when there is concentration of CO2 ranging from 5-10% despite the availability of the requirement of their oxygen.

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