ELECTROLYTE

ELECTROLYTE

Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion also More »

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

LIST OF TEST UNDER E/U/CREATININE Sodium (Na) Potassium (K) Chloride (Cl) Bicarbonate (HCO3) Urea (U) Creatinine (Cr) Glucose (Gluc)   ESTIMATION OF SODIUM & POTASSIUM There are three method used for the More »

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASIUM ESTIMATION REAGENT   A STOCK SODIUM CHLORIDE STANDARD (1.0M) COMPOSITION Sodium chloride 45g Distilled water 1litre PREPARATION Dissolved 58.45g of sodium chloride in 20ml of distilled water, make up the solution to More »

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY REAGENT DILUTING FLUID COMPOSITION Glacial acetic acid 100ml Concentrated nitric acid 6.4ml Deionized water 1litre PREPARATION Mixed 100ml of glacial acetic acid with 200ml of deionized More »

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

ESTIMATION OF PLASMA/SERUM BIOCARBONATE REAGENT NORMAL SALINE COMPOSITION Saline                                           1g Distilled water                            100ml PREPARATION Dissolved 1g of Sodium chloride in 100ml of 100ml water 01N (HCL) HYDROCHLORIC ACID (NaOH) SODIUM HYDROXIDE 01N More »

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA Almost half of non proteinous nitrogen substance found in the blood is the constituent of urea. Urea is a product of amino acid that is produce when amino More »

container to be used collect dample for blood urea Nitrogen Test

container to be used collect dample for blood urea Nitrogen Test

SAPMLE USE TO CARRY OUT THE TEST SPECIMEN: whole blood can be used to perform blood urea nitrogen. Since whole blood can be used, then EDTA container can be use to collect More »

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD REAGENT BUFFERED UREASE REAGENT COMPOSITION EDTA (Ethylene diaminetetracetic acid)                     1.0g Urease                                                                           150mg Distilled water                                                              100ml PREPARATION Dissolved 1.0g of EDTA (Ethylene diaminetetracetic acid), and 150mg More »

Laboratory Examination Of Creatinine

Laboratory Examination Of Creatinine

ESTIMATION OF CREATININE Creatinine is a nitrogenous product that is produced when metabolism of creatine in the skeletal and muscle. They are filter by the kidney and excreted in the urine. REAGENT More »

GLOCOMETER AND IT USES

GLOCOMETER AND IT USES

Glucometer can be define as instrument use for measuring the blood sugar level of a patient. The advent of glucometer come as result of scientist try to finding quick way to estimate More »

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

ELECTROLYTE Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion More »

cause of male infertility and laboratory investigation

cause of male infertility and laboratory investigation

seminal fluid analysis Seminal fluid analysis is one of the important investigation of male in fertility in medical laboratory. The often requested for men that have issue of getting a woman pregnant. More »

Effect of alcohol on the body system

Effect of alcohol on the body system

Effect of alcohol on the body system Digestive and endocrine glands: Drinking too much alcohol can cause abnormal activation of digestive enzymes produced by the pancreas. Buildup of these enzymes can lead More »

SEMINAL FLUID ANALYSIS

SEMINAL FLUID ANALYSIS

Practical step of seminal fluid analysis EXAMINATION PARAMETER MACROSCOPIC EXAMINATION Time of arrival Appearances of the specimen Viscosity/liquefaction Volume of the specimen MICROSCOPIC EXAMINATION MOTILITY Quantitative motility Qualitative motility Sperm count HOW More »

 

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

REAGENT

  1. BUFFERED UREASE REAGENT

COMPOSITION

EDTA (Ethylene diaminetetracetic acid)                     1.0g

Urease                                                                           150mg

Distilled water                                                              100ml

PREPARATION

Dissolved 1.0g of EDTA (Ethylene diaminetetracetic acid), and 150mg urease in 70ml of distilled water, adjust the solution to PH 6.5 and make up the solution to 100ml with distilled  water.

The solution is stable for 1 month.

  1. PHENOL COLOUR SOLUTION

COMPOSITIION

Analytical grade phenol                                    50g

Analytical grade sodium nitroprusside              250mg

Distilled water                                                   1litre

PREPARATION

STEP 1

SOLUTION 1

Dissolved 50mg of Analytical grade phenol in 400ml of distilled water.

STEP 2

SOLUTION 2

Dissolved 250mg of Analytical grade sodium nitroprusside in 50ml of distilled water.

STEP 3

Mixed solution 1 with solution 2 and make it up to the final volume of 1litre with distilled water.

Store the solution in a dark brown bottle and it should be kept away from light, store at 4ºC it is stable for 2 month.

  1. ALKALINE HYPOCHLORITE REAGENT

COMPOSITION

Sodium hydroxide                                        25g

Sodium hypochlorite                                     2.1g

Distilled water                                               1lire

PREPARATION

STEP 1

SOLUTION 1

Dissolved 25g of Sodium hydroxide in 400ml of distilled water

STEP 2

SOLUTION 2

Dissolved 2.1g of Sodium hypochlorite in 100ml of distilled water

STEP 3

Mixed solution 1 with solution 2 and make it up to the final volume of 1litre with distilled water.

Store the solution in a dark brown bottle and it should be kept away from light, store at 4ºC it is stable for 2 month.

  1. UREA STANDARD (10mmol/L) REAGENT

COMPOSITION

Urea                                              600mg

Distilled water                              1litre

ChloroformPREPARATION

Dissolved 600mg of Urea in a quantity of distilled water, and make up the solution to 1litre with distilled water, add a few drop of chloroform as a preservative.

READ  LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

METHOD

STEP 1

Set up three tubes as follow;

 

REAGENT TEST TUBE STD TUBE BLK TUBE
Plasma 20µl    
Buffer urea reagent 200µl 200µl 200µl
Standard   20µl  
Distilled water     20µl

STEP 2

Incubate all the tube using water bath at 37ºC for 15 minute

Remove all the tube from the water bath to room temperature, add 1.0ml phenol colour reagent to each tube, and shake each of the tube gently to mix very well.

Add 1.0ml hypochrite to each tube and shake to mix.

 

STEP 3

Incubate all the tube again at 37ºC for 20 minutes

STEP 4

Remove all the tube from water bath; add 5ml of distilled water to each tube

Read absorbance of the standard and the test, using the blank to set the machine to zero, record your reading.

 

CALCULATION

Ab. Test

Urea plasma Con. In mol/l =  ———————   x 10mmol/l

Ab. Std

 

 

Leave a Reply