ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

REAGENT

  1. DILUTING FLUID

COMPOSITION

Glacial acetic acid 100ml

Concentrated nitric acid 6.4ml

Deionized water 1litre

PREPARATION

Mixed 100ml of glacial acetic acid with 200ml of deionized water, add 6.4ml of concentrated nitric acid, and make it up to 1lite with deionized water.

  1. INDICATOR SOLUTION

COMPOSITION

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Unflavored gelatin 6.0g

Thymol blue 0.1g

Thymol 0.1g

Deionized water 1litre

PREPARATION

Dissolved 6.0g of unflavored gelatin, 0.1g thymol blue, and 0.1g hymol in 500ml of dionized water, make up the solution to 1litre with dionized water by gentle heating, when the it fully dissolved, bring the solution to room temperature and make it up 1litre with deionized water. Store at 2-8ºC.

  1. STANDARD CHLORIDE SOLUTION 100mmol/L
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COMPOSITION

Sodium chloride 5.85g

Deionized water 100ml

PREPARATION

Dissolved 5.85g of Sodium chloride in 100ml of dionized water

METHOD

  1. Measure 0.1ml of serum in test tube
  2. Add 10ml of reagent 1 to the test tube
  3. Add 2-3 drop of reagent 2
  4. Deep in the electrode in the test tube, switch on the machine
  5. Start the digital display
  6. There will be a stop of the display reading on machine when the concentration of chloride is indicated
  7. The same procedure should be repeated with 0.1ml of reagent 3 in other to be sure of the accuracy of the result.
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NORMAL VALUE

Serum/chloride 75-105mmol/L

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