Clinical Chemistry

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASIUM ESTIMATION REAGENT   A STOCK SODIUM CHLORIDE STANDARD (1.0M) COMPOSITION Sodium chloride 45g Distilled water 1litre PREPARATION Dissolved 58.45g of sodium chloride in 20ml of distilled water, make up the solution to 1litre with distilled water. ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY REAGENT B STOCK POTASSIUM CHLORIDE STANDARD (1.0M) COMPOSITION Potassium chloride 55g Distilled water […]

Clinical Chemistry

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY REAGENT DILUTING FLUID COMPOSITION Glacial acetic acid 100ml Concentrated nitric acid 6.4ml Deionized water 1litre PREPARATION Mixed 100ml of glacial acetic acid with 200ml of deionized water, add 6.4ml of concentrated nitric acid, and make it up to 1lite with deionized water. INDICATOR SOLUTION COMPOSITION Unflavored gelatin 6.0g Thymol […]

Clinical Chemistry

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

ESTIMATION OF PLASMA/SERUM BIOCARBONATE REAGENT NORMAL SALINE COMPOSITION Saline                                           1g Distilled water                            100ml PREPARATION Dissolved 1g of Sodium chloride in 100ml of 100ml water 01N (HCL) HYDROCHLORIC ACID (NaOH) SODIUM HYDROXIDE 01N COMPOSITION Distilled water Sodium hydroxide   PREPARATION Dissolve 0.1NaOH in distilled water, make 1: 10 dilution.     INDICATOR SOLUTION COMPOSITION Phenol red                             […]

Clinical Chemistry

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA Almost half of non proteinous nitrogen substance found in the blood is the constituent of urea. Urea is a product of amino acid that is produce when amino acid is broken down by the liver. ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD The major excretory product of protein when it is broken […]

Clinical Chemistry

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD REAGENT BUFFERED UREASE REAGENT COMPOSITION EDTA (Ethylene diaminetetracetic acid)                     1.0g Urease                                                                           150mg Distilled water                                                              100ml PREPARATION Dissolved 1.0g of EDTA (Ethylene diaminetetracetic acid), and 150mg urease in 70ml of distilled water, adjust the solution to PH 6.5 and make up the solution to 100ml with distilled  water. The […]

Clinical Chemistry

Laboratory Examination Of Creatinine

ESTIMATION OF CREATININE Creatinine is a nitrogenous product that is produced when metabolism of creatine in the skeletal and muscle. They are filter by the kidney and excreted in the urine. REAGENT SATURATED PICRIC ACID SOLUTION COMPOSITION Picric acid Distilled water PREPARATION Place an excess of picric acid in an amber colour bottle containing distilled […]