ELECTROLYTE

ELECTROLYTE

Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion also More »

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

ELECTROLYTE UREA AND CTREATININE TEST (E/U/CREATININE)

LIST OF TEST UNDER E/U/CREATININE Sodium (Na) Potassium (K) Chloride (Cl) Bicarbonate (HCO3) Urea (U) Creatinine (Cr) Glucose (Gluc)   ESTIMATION OF SODIUM & POTASSIUM There are three method used for the More »

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASSIUM ESTIMATION

SODIUM/POTASIUM ESTIMATION REAGENT   A STOCK SODIUM CHLORIDE STANDARD (1.0M) COMPOSITION Sodium chloride 45g Distilled water 1litre PREPARATION Dissolved 58.45g of sodium chloride in 20ml of distilled water, make up the solution to More »

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY

ESTIMATION OF PLASMA/SERUM CHLORIDE WITH COULOMETRY REAGENT DILUTING FLUID COMPOSITION Glacial acetic acid 100ml Concentrated nitric acid 6.4ml Deionized water 1litre PREPARATION Mixed 100ml of glacial acetic acid with 200ml of deionized More »

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

LABORATORY ESTIMATION OF PLASMA/SERUM BIOCARBONATE

ESTIMATION OF PLASMA/SERUM BIOCARBONATE REAGENT NORMAL SALINE COMPOSITION Saline                                           1g Distilled water                            100ml PREPARATION Dissolved 1g of Sodium chloride in 100ml of 100ml water 01N (HCL) HYDROCHLORIC ACID (NaOH) SODIUM HYDROXIDE 01N More »

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA

BLOOD ESTIMATION OF UREA Almost half of non proteinous nitrogen substance found in the blood is the constituent of urea. Urea is a product of amino acid that is produce when amino More »

container to be used collect dample for blood urea Nitrogen Test

container to be used collect dample for blood urea Nitrogen Test

SAPMLE USE TO CARRY OUT THE TEST SPECIMEN: whole blood can be used to perform blood urea nitrogen. Since whole blood can be used, then EDTA container can be use to collect More »

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD

ESTIMATION OF PLASMA/SERUM UREA USING BERTHELET METHOD REAGENT BUFFERED UREASE REAGENT COMPOSITION EDTA (Ethylene diaminetetracetic acid)                     1.0g Urease                                                                           150mg Distilled water                                                              100ml PREPARATION Dissolved 1.0g of EDTA (Ethylene diaminetetracetic acid), and 150mg More »

Laboratory Examination Of Creatinine

Laboratory Examination Of Creatinine

ESTIMATION OF CREATININE Creatinine is a nitrogenous product that is produced when metabolism of creatine in the skeletal and muscle. They are filter by the kidney and excreted in the urine. REAGENT More »

GLOCOMETER AND IT USES

GLOCOMETER AND IT USES

Glucometer can be define as instrument use for measuring the blood sugar level of a patient. The advent of glucometer come as result of scientist try to finding quick way to estimate More »

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

FUNCTION OF ELECTROLYTE IN THE BODY SYSTEM

ELECTROLYTE Electrolyte is a substance which when melted, will facilitate free flow of electricity. The substance form two charge ions, which are positively charge ions also called cations and negatively charge ion More »

cause of male infertility and laboratory investigation

cause of male infertility and laboratory investigation

seminal fluid analysis Seminal fluid analysis is one of the important investigation of male in fertility in medical laboratory. The often requested for men that have issue of getting a woman pregnant. More »

Effect of alcohol on the body system

Effect of alcohol on the body system

Effect of alcohol on the body system Digestive and endocrine glands: Drinking too much alcohol can cause abnormal activation of digestive enzymes produced by the pancreas. Buildup of these enzymes can lead More »

SEMINAL FLUID ANALYSIS

SEMINAL FLUID ANALYSIS

Practical step of seminal fluid analysis EXAMINATION PARAMETER MACROSCOPIC EXAMINATION Time of arrival Appearances of the specimen Viscosity/liquefaction Volume of the specimen MICROSCOPIC EXAMINATION MOTILITY Quantitative motility Qualitative motility Sperm count HOW More »

 

Sickle Cell test Using Sodium metabisulphate

Examination of sickle cell is one of the important diagnosis in the medical laboratory, this test is carry on a patient suspected with sickle cell syndrome. The examination can also be conducted where there is malfunction of electrophoretic chromatographic hemoglobin fraction in the position of Hb S. a lot of this investigation has been explained, many kit produce for rapid detection is available. But where are going focus on another simple and easy method of investigating sickle cells that is proven reliable.

Mechanism behind the investigation

Sodium metabisulphate limit tension of oxygen, that turn bring out the abnormal shape of blood different biconcave shape of normal blood.

Material

Bright field binocular Microscope

Cover slip

Glass Slide

Beaker

Reagent

Distilled water

Sodium metabisulphate

Reagent preparation: measure accurately 0.2mg of sodium metabisulphate in 10ml of distilled water, stir the mixture until it all dissolved.

Note that fresh sample reagent must be prepare anytime it must be use.

Method

  1. Mix 1 drop of blood with 1 drop of 2% sodium metabisulphate on a microscopic slide
  2. Cover with cover slip, and the use candle wax to seal the edge of the slide in contact with cover slip
  3. Allowed the prepare sample to stand from 1 to 4 hours
  4. Examine under microscope with wet objective lens,

In a sickle cells positive patient, sickle shape of red blood cell will appear like half-moon as shown in the picture below. But on some condition when the sample will not be examine on due time, if it may take 24 hours, the slide should be store in on a moist petri dish.

READ  Hematology

If the edge of the slide in contact with coverslip is not seal properly or there is reduction in the potency of sodium metabisulphite, false positive result may occur. cause of male infetily and laboratory investigation

Also bear in mind that, positive test never isolate sickle cell trait in a patient, as such special care should be adhere to by examine the preparation carefully at the edge near the coverslip.

One Response to Sickle Cell test Using Sodium metabisulphate

  1. omokoshaban says:

    love your work thanks so much

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